Program to find gene copy number in genome?

I am trying to find out how many copies of a gene that I have primers for are in a training set of genomes. I would like program where I enter the genome and the primer (that has degenerate...

15 November 2020 4,523 3 View

Do you have an endnote style for Marine Ornithology?

Any seabird folks out there have an endnote style file for Marine Ornithology? Nothing available online, trying to avoid creating one from scratch - thanks! Perhaps we can make the file publicly...

01 November 2020 9,642 7 View

Storing environmental data with PostgreSQL?

I am wondering if anyone uses postgresql to store data collected by continuous sensors and if there is a pipeline that makes this easier? Also, any other general tips with postgresql would be very...

18 August 2020 939 2 View

Is it necessary to do elution of proteins from Polyacrylamide gel?

Dear researchers, I would like to ask is it necessary to do Elution of proteins from Polyacrylamide gel before sending the protein sample to Nano-LC-TOF-MS Analysis? This is what I plan, I would...

01 August 2020 3,680 3 View

Aside from Mendeley, what other tools/apps can help researchers organize their citations and references?

My question is on looking for suggestions on how citations and references can be easier to generate. Thanks for the response. Hop your sharings could help me.

31 May 2020 5,026 14 View

Hi, i am conducting a legal research on; why people in Ghana are reluctant to commence tort actions like battery in court?

would be very grateful if some recommendations are made on the relevant cases or articles to read to help. thank you.

20 April 2020 4,801 3 View

Regarding on metabolomic?

Dear researchers, May I know how to know what metabolites will be produced by the cells from experiment (Wetlab): 1) Cell (cell culture) 2) Cell infected with virus 3) Cell treated with plant...

31 March 2020 5,865 3 View

How to make elution buffer for SDS-PAGE?

Dear researchers, How to make elution buffer for SDS-PAGE? There is a lot of protocol to make elution buffer, but they didn't show the amount of each ingredient needed. thank you.

25 March 2020 8,333 4 View

Elution of protein from SDS PAGE ?

Dear researchers, May I know do anyone can suggest the protocol of protein elution from SDS PAGE ? Any simple and inexpensive methods? Thank you.

24 March 2020 2,073 13 View

Regarding on Proteomics ?

Dear researchers, I'm currently doing a study regarding on antiviral mechanism of selected plant using metabolomic approach. Plant extract (fruit) will be used and the virus model will be used is...

21 March 2020 5,885 2 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View