Interpolation of product formation in biocatalysis using integrated Michaelis-Menten equation?

I want to interpolate the amount of product formed (as concentration or % of conversion) vs reaction time in a biocatalysis process. The fitting equation should have as (y) the amount of product...

31 December 2017 7,258 3 View

Which artificial electron acceptor could be used instead of cytochrome b?

04 May 2015 680 2 View

What can be used to block E. coli cytochrome b?

04 May 2015 6,686 2 View

Is there an "acid catalase"?

10 November 2014 3,758 8 View

How many H-bonds can the N atom of the peptide bond form (as a donor and an acceptor)?

For sure the nitrogen atom of the peptide bond can act as a donor of one H-bond. Can the lone pair of the same atom also be used to accept a H-bond?

03 April 2014 8,207 1 View

Bioinformatic tools for prediction of protein solubility?

Which are the best online servers for the prediction of the solubility of a recombinant protein expressed in E. coli?

10 November 2013 781 5 View

A simple way to calculate the binding energy between a ligand and a protein?

I would like to have an estimate of the binding energy due to the non-bonded interactions between a ligand and a binding site on a protein. I used to get this data from the output of docking...

07 August 2013 2,428 21 View

Is there a way to reduce in the results of BLAST search the number of sequences that are too similar among each other?

Due to the large amount of sequence data deposited in the databanks, when I perform a BLAST search, I often end up with a list of proteins whose sequence is very similar among each other. I'm...

05 June 2012 1,538 18 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View