Interpolation of product formation in biocatalysis using integrated Michaelis-Menten equation?

I want to interpolate the amount of product formed (as concentration or % of conversion) vs reaction time in a biocatalysis process. The fitting equation should have as (y) the amount of product...

31 December 2017 7,258 3 View

Concentration of exogenous H2O2 in E. coli medium?

I would like to induce production of ROS-scavenging enzyme in E. coli before induction of my recombinant protein. Which is the final concentration of H2O2 I should add to the medium? Should I add...

09 October 2017 4,155 2 View

Which artificial electron acceptor could be used instead of cytochrome b?

04 May 2015 680 2 View

Is there an "acid catalase"?

10 November 2014 3,758 8 View

How many H-bonds can the N atom of the peptide bond form (as a donor and an acceptor)?

For sure the nitrogen atom of the peptide bond can act as a donor of one H-bond. Can the lone pair of the same atom also be used to accept a H-bond?

03 April 2014 8,207 1 View

Bioinformatic tools for prediction of protein solubility?

Which are the best online servers for the prediction of the solubility of a recombinant protein expressed in E. coli?

10 November 2013 781 5 View

A simple way to calculate the binding energy between a ligand and a protein?

I would like to have an estimate of the binding energy due to the non-bonded interactions between a ligand and a binding site on a protein. I used to get this data from the output of docking...

07 August 2013 2,428 21 View

Is there a way to reduce in the results of BLAST search the number of sequences that are too similar among each other?

Due to the large amount of sequence data deposited in the databanks, when I perform a BLAST search, I often end up with a list of proteins whose sequence is very similar among each other. I'm...

05 June 2012 1,538 18 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View