9 Questions 14 Answers 0 Followers
Questions related from Gianluca Molla
I want to interpolate the amount of product formed (as concentration or % of conversion) vs reaction time in a biocatalysis process. The fitting equation should have as (y) the amount of product...
01 January 2018 7,300 3 View
I would like to induce production of ROS-scavenging enzyme in E. coli before induction of my recombinant protein. Which is the final concentration of H2O2 I should add to the medium? Should I add...
10 October 2017 4,213 2 View
I'm working with a purified membrane protein (from E. coli) that is supposed to transfer electrons to cytochrome b. Which compound could I use instead of the putative physiological acceptor...
05 May 2015 752 2 View
I'm working with an enzyme that probably reduces cytochrome b of E. coli. How can I inhibit cytochrome b so that the electron transfer would be blocked? Literature search showed several ihibitors...
05 May 2015 6,715 2 View
I'm looking for a catalase that is able to work at low pHs (about pH 2-3). From PubMed and Brenda database I was not able to find such a peculiar catalase. Probably this low pH is not compatible...
11 November 2014 3,793 8 View
For sure the nitrogen atom of the peptide bond can act as a donor of one H-bond. Can the lone pair of the same atom also be used to accept a H-bond?
04 April 2014 8,249 1 View
Which are the best online servers for the prediction of the solubility of a recombinant protein expressed in E. coli?
11 November 2013 847 5 View
I would like to have an estimate of the binding energy due to the non-bonded interactions between a ligand and a binding site on a protein. I used to get this data from the output of docking...
08 August 2013 2,637 21 View
Due to the large amount of sequence data deposited in the databanks, when I perform a BLAST search, I often end up with a list of proteins whose sequence is very similar among each other. I'm...
06 June 2012 1,583 18 View
