Interpolation of product formation in biocatalysis using integrated Michaelis-Menten equation?

I want to interpolate the amount of product formed (as concentration or % of conversion) vs reaction time in a biocatalysis process. The fitting equation should have as (y) the amount of product...

31 December 2017 7,258 3 View

Concentration of exogenous H2O2 in E. coli medium?

I would like to induce production of ROS-scavenging enzyme in E. coli before induction of my recombinant protein. Which is the final concentration of H2O2 I should add to the medium? Should I add...

09 October 2017 4,155 2 View

Which artificial electron acceptor could be used instead of cytochrome b?

04 May 2015 680 2 View

What can be used to block E. coli cytochrome b?

04 May 2015 6,686 2 View

Is there an "acid catalase"?

10 November 2014 3,758 8 View

How many H-bonds can the N atom of the peptide bond form (as a donor and an acceptor)?

For sure the nitrogen atom of the peptide bond can act as a donor of one H-bond. Can the lone pair of the same atom also be used to accept a H-bond?

03 April 2014 8,207 1 View

A simple way to calculate the binding energy between a ligand and a protein?

I would like to have an estimate of the binding energy due to the non-bonded interactions between a ligand and a binding site on a protein. I used to get this data from the output of docking...

07 August 2013 2,428 21 View

Is there a way to reduce in the results of BLAST search the number of sequences that are too similar among each other?

Due to the large amount of sequence data deposited in the databanks, when I perform a BLAST search, I often end up with a list of proteins whose sequence is very similar among each other. I'm...

05 June 2012 1,538 18 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View