I am working on agrobacterium mediated potato transformation using cultivar Bintje for tissue culture. I am not sure about the concentration of adenine sulphate. The range in growth medium of explants is from 20-400 mg/L. De Block used 40 mg/L and in another paper they used 400 mg/L, which I think is too much. Can you please help me that what concentration I should use especially for potato cultivar Bintje? Thanking you in advance
Hi, Ahmad,
I would suggest to use 40 mg/l. 400 mg/l is really too much, and this concentration have adverse effect for sure. best wishes!
Thank you very much Pasternak for your suggestion, but the problem lies here that the protocol I am following for cultivar Bintje (I am using Bintje for explants too) used 0.04% of adenine. Please advise.
Thanks again, here is the paper.
Article Rapid regeneration of stable transformants in cultures of po...