Thawing of GV3101 agrobacterium competent cells took 50 minutes on ice after taking out from -80c I don't know why it took so long. Any suggestions?

Direct Agrobacterium Transformation Of GV3101(PMP90RK) With PLH Vectors Thaw the competent cells carefully on ice (here it took 50 minutes). Add 0,1-1 µg of DNA to the cells and mix.Freeze the...

19 January 2016 1,023 2 View