Sajjad Ahmad

2 Questions 5 Answers 0 Followers

Questions related from Sajjad Ahmad

Concentration of adenine sulphate range is quite wide from 20-400 mg/L in potato tissue culture. Any suggestion for potato cultivar Bintje?

I am working on agrobacterium mediated potato transformation using cultivar Bintje for tissue culture. I am not sure about the concentration of adenine sulphate. The range in growth medium of...

10 May 2016 8,547 4 View

Thawing of GV3101 agrobacterium competent cells took 50 minutes on ice after taking out from -80c I don't know why it took so long. Any suggestions?

Direct Agrobacterium Transformation Of GV3101(PMP90RK) With PLH Vectors Thaw the competent cells carefully on ice (here it took 50 minutes). Add 0,1-1 µg of DNA to the cells and mix.Freeze the...

19 January 2016 1,105 2 View