Compatible of QIAseq Stranded Total RNA Lib Kit and Ribominus Transcriptome Isolation Kit?

29 October 2019 1 8K Report

Dear all,

I'm going to perform RNA-Seq with a QIAseq Stranded Total RNA Lib Kit and depletion with Ribominus Transcriptome Isolation Kit/ Ribominus concentration Kit. Does anyone have experience with this kits? Are these kits compatible?

Thanks in advance.

Abhijeet Singh

There need not be be any compatibility between the kits. You can isolate total RNA with any kit and deplete rRNA with any kit available on market.

However, in my experience, RiboMinus from Thermo-Fisher is not at depleting rRNA. THe kit claims 95% efficiency, but the further analysis with bioanalyzer gives different figures and in RNA-seq data. So, in my personal opinion, I would not recommend RiboMinus now.

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