Does anyone know of a company (preferably in the US) that will analyze riboflavin levels in human urine via HPLC?
I've called a few companies that offer HPLC services but none will do human urine.
Thanks for the help!
You should look at clinical testing companies. Urine would be a normal sample for them, and testing vitamin levels would be routine. I'm not sure if clinical vitamin testing relies on HPLC, but if you got the data from a clinical method (which comes with trustworthy accuracy by definition) maybe you don't need HPLC data specifically?
ARUP comes to mind as a testing possibility (aruplab.com). I think Mayo's clinical lab also offers research support. Maybe an easy first try would be your local medical school or hospital --> contact the Director of the clinical laboratory in the hospital and they might help you out.
Probably SGS. Urine should be no different than water as long as you deproteinize beforehand (maybe ACN). Then maybe SPE.
See and adapt the attached article;
Article Rapid determination of vitamin B-2 (riboflavin) in plasma by HPLC
I'd be happy to give you a quotation. Please see my contact information on my Web site www.austinanalytical.com
- Badges
- Science method