What econometric models can be used for testing the effect of economic reforms on economic growth?

I am writing my dissertation about the effects of economic reforms on economic growth in the case of Egypt in 2016 and I need suggestions for what econometric models are usually used in testing...

06 January 2021 2,943 6 View

When the thin film peels off from the substrate?

When the thin film peels off from the substrate?

23 September 2020 286 5 View

What are the most important quantifiers that are used by the university Students of English?

Using Quantifiers in English University Students: Problems a...

24 December 2019 8,632 2 View

Why increasing the Neural Network layer size saturates the cost?

I wrote a NN code in python, tensorflow. The network has two layers. My dataset has 28000 samples (25 feature/sample). When i run the code for a small layer sizes (around 10 neurons/layer) the...

16 January 2019 1,393 4 View

How are densely connected convolutional networks?

convolution network an be substantially deeper, more accurate and efficient to train if they contain shorter connections between layers close to the input and those close to the output .how are...

09 March 2018 8,096 2 View

Where is the best source for proteins primary structures and corresponding secondary structure ?

I am trying to find away to predict 2ry structure of protein" helices or sheets" from the amino acid sequence by studying the amino acids doublets and triplets so I can predict the helix if a...

21 December 2017 4,126 4 View

What is the reasons of voltage sag in pmsg wind turbine connected to the grid?

What is the reasons of voltage sag in pmsg wind turbine connected to the grid?

05 November 2017 5,911 2 View

How to calculate the fluorescent decay rates (radiative and non-radiative) of two emitting species (flourophore) in solution i.e. two lifetimes ?

So I have the PL spectra and lifetime transients for a fluorophore in solution (Perylene based polymer in Chloroform) and so by fitting the transients I am able to extract 2 lifetimes one...

03 July 2017 3,346 4 View

Simple random sampling in online surveys?

11 March 2017 4,710 14 View

Are there any new therapeutic options for head and neck cancer rather than classic surgical resection with or without chemoradiation?

Head and neck cancer is still a fatal disease particularly inoperable metastatic types. Cancer pathology regarding oncogenes & stem tumor cells is in progress, but on the other side, therapeutic...

01 July 2014 3,805 18 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View