Cloning of poly A tail?

19 October 2023 0 5K Report

Hi,

I want to add Poly A Tail at the end of my construct after 3'UTR so that I do not have to do Poly A Tailing every time I am making RNA and doing injections in Parhyale.

Can someone guide me on how to do it? Is it normal cloning of 200nt A, I can do it via Gibson?

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