Adding Poly A tail?

11 October 2023 1 351 Report

I want to add poly A tail at the end of 3’UTR so that I don’t have to do poly-A tailing every time I make RNA. Please let me know how I can do it. It should be poly A 200nt correct? should I make a g-block and clone it?

Yoav Lubelsky

g-block or similar will work. The one I used has a 30A-linker-70A structure.

Our lab IVT template plasmids that include either GFP or luciferase followed by a polyA are avalible on addgene.

https://www.addgene.org/browse/article/28238347/

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