Can anyone help me to remove a hydrophobic peptide from C18 column?

I have injected a hydrophobic peptide into analytical C18 column and i have tried to cleaning the column applying a lot of protocols using acetonitrile, methanol, isopropanol, but in the blank...

05 January 2021 3,305 3 View

Could you help with a doubt regarding UDF velocity inlet profile with multiphase mixture (Ansys Fluent)?

We are two undergraduate students seeking for help!!! We are trying to simulate a laminar flux through a 3D pipe (25 cm long, 2 cm of diameter) consisting of a multiphase flux (air and water). We...

23 November 2020 3,918 3 View

What is the role of Digital Services in Crisis, Disaster, and Emergency Situations?

06 January 2020 7,241 4 View

How to dissolve Ibuprofen?

I need to obtain IBU aqueous solution. Some papers report that they dissolve it in water/methanol (90/10). But it did not work. What to use to dissolve it? Some buffer or NaOH? Or maybe some salt....

05 May 2019 6,575 1 View

How come conductivity increased at very low concentrations of CuSO4 after sorption of activated carbon?

I was investigating the sorption of activated carbon on different concentrations of copper(II)sulfate and came across rather unexpected results where the conductivity increased at 0,00001 , 0,0001...

24 November 2018 6,855 3 View

What is a good cycling method for 27F and 519R primers? Tm is 56 and 66, can they be used as a pair?

I'm trying to generate a smaller fragment of 16S from a water sample, so it can be NGS amplicon sequenced. The limit of that service is about 500bp.

14 March 2018 8,032 2 View

Can I use aneuploidy for crossing?

Dear Collegues, we are going to make segregating population of tea plant (camellia sinensis) for genetic research. we need 200 progenies. Can we use aneuploid cultivar as one of the parents to...

19 October 2017 8,451 3 View

Protocol of fungal dna extraction from plant leaf tissue?

Dear collegues, Can we use protocols of DNA extraction from plant tissues (from leaves) to extract DNA of fungal pathogen which lives inside of tissue? If yes, will the quantity of the pathogen's...

11 April 2017 8,448 31 View

If I have low bootstrap values in a ML tree, does it mean that there is no separation between different genera?

I have a maximum likelihood tree (18S and COI) for a nematode family which contains 4 different genera. The bootstrap values were very low and all the 4 genera are intermingled in both trees with...

27 June 2016 593 5 View

Why does the PCR not work occasionally?

Dear collegues! we extracted RNA from apple peel. We need clean RNA without DNA contamination, so we check our RNA samples by PCR. Samples are fresh. and reagents are the same. BUT one time PCR...

24 March 2015 4,855 12 View

Apricot database - where to search?

I work in apricot research and we have a breeding program for abiotic stress tolerance. I collect data from the cultivars i could use in this program individually, but there are several hundred...

01 February 2021 386 3 View

How to generate ECFP hashing folding data?

I'm trying to convert chemical structures to ECFP data. Buy, I have a problem about folding step. I understand all of the process about generating ECFP data though D. Rogers and M. Hahn's paper...

09 January 2021 7,454 2 View

The use of Orange, a high level tookit for Machine Learning, data mining and data science for scientific research or just teaching?

Related to data mining/data science, I recently discovered Orange (https://orange.biolab.si/) a high level very powerful free toolkit. Using it is very simple and you can test different models in...

09 December 2020 8,832 4 View

How do I retrieve every CID from pubchem?

Hi all, I guess there are probably several ways of doing this. I'm looking to get access to every Pubchem CID. All compounds are published for download, but the files are quite large in size and...

01 November 2020 8,612 3 View

What are the genetic/physiological differences between deep water rice cultivars of North-Eastern India from that of South East Asian Countries?

Deepwater rice cultivars found in various regions around the world have their own differences. Please mention how the deepwater rice cultivars of North-Eastern India varies from that of Southeast...

23 October 2020 1,333 3 View

How can I get help to identify a species of Coniopterygidae from Peru?

I found a species of Coniopterygidae preying on eggs and nymphs of Aleurothrixus floccosus in a lemon tree. I have followed its biology and have pictures of eggs, larvae, pupae and adults. With my...

16 October 2020 6,421 2 View

Does citrus plant can be effective as Nanopesticides?

good day everyone may i ask a request if there are any study about citrus plant that been used as nanopesticides since google only show a nanoformulation and nanoparticles that uses citrus plant....

14 October 2020 6,898 4 View

How to rank data and transform responses of a variable in SPSS?

So i have data something like this in SPSS at the moment: case 1: orange, bananas, mangoes case 2: bananas, oranges, mangoes So the first respondent ranked orange as best fruit whilst second...

07 September 2020 8,305 5 View

How much raw materials are required for producing 1 kg of final products for each of the following?

How much raw materials are required for producing 1 kg of final products for each of the following? 1. Orange jam 2. Orange Jelly 3. Orange Marmalade 4. Tomato sauce 5. green Chilli Sauce 6....

01 September 2020 6,126 5 View

What systematic experiment will help determine whether or not a range of mutants with similar phenotype are derived from a common ancestor-cultivar?

Several soybean cultivars were mutagenized to produce many lines of M0, M1, M2 etc. In order to determine the genetic basis of an observed phenotype, an M0 level mutant was hybridized with its...

09 August 2020 9,224 3 View