I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for the gene. I have tested and performed some pilot experiments with HEK293T-D10 cells. However, would be interested to use neuronal cell lines.
Any recommendations? I am not an experienced cell biologist. Thus, the cell line should be easy to maintain and cultivate.
Thanks in advance.
Enkhtuya Radnaa
PC12 is one of the commonly used neuronal cell lines that I used to work with. This is progenitor cells, you can add NGF (neuronal growth factor) to differentiate them in the culture.
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