I would like to do the natural transformation of BT-1 strain, the literature said I can use 50 ul chitin (1mM), does any expert know how to make a chitin solution. because chitin is too stable to dissolve into water.
Chapter Solubility of Chitin: Solvents, Solution Behaviors and Their...
Check https://www.intechopen.com/chapters/57402
Also check https://www.researchgate.net/publication/323777293_A_Simple_and_Effective_Method_for_Preparation_of_Chitosan_from_Chitin
Check this paper also
https://www.frontiersin.org/articles/515905
Hi Dr Chenhao li . See the following useful link: https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/chitin
Kindly see also the following useful RG link: Article Chitin and Chitosan Preparation from Marine Sources. Structu...
Also see the following use link: Article Preparation and Solubility in Acid and Water of Partially De...
thanks very much for the kind help @Michael G. Weller@Chinaza Godswill Awuchi@Kiprotich Kiptum@Aref Wazwaz
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