Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Hello everyone, good day! In the process of deproteinization of samples to obtain chitin, can I make use of a phenol as a deproteinizing agent?

I would like to know what other reagents can be used to obtain chitin by deproteinizing a sample. Commonly found on articles is the employment of NaOH concentrations as well as KOH. I would like...

16 October 2020 537 6 View

What are the equation and conversion factor to estimate protein content from mushroom?

Total nitrogen was calculated with Kjeldahl method, I found different equations and factors ''Total nitrogen content of shrimp waste was determined using the Kjeldahl method. Separately, pure...

17 August 2020 4,572 9 View

Why Calcofluor white stain fungal cells walls?

Calcofluor white (CW) is recommended to stain plant cell wall that has B1-4 Glucan linkage. On the other hand fungal chitin has Different composition. Thus, I was wondering if anyone can give me...

01 February 2020 6,120 2 View

Can anyone suggest a suitable deuterated solvent to dissolve chitin for NMR analyses?

Can anyone suggest a suitable deuterated solvent to dissolve chitin for NMR analyses?

11 December 2019 9,695 10 View

How can I easily determine the purity of my chitin ?

I extracted chitin with chemical method, now I would like to know approximatly the efficiency of my method and know for example how much proteins and minerals are left. I would like to find a...

10 November 2019 8,937 3 View

What is an appropriate solvent for chitin?

I want to inject the chitin solution into the foliage of lettuce and measure a possible difference is reactive oxygen species production. Conventional solvents are often toxic or corrosive which...

10 November 2019 743 3 View

What is an appropriate solvent for chitin?

I want to inject the chitin solution into the foliage of lettuce and measure a possible difference is reactive oxygen species production. Conventional solvents are often toxic or corrosive which...

06 November 2019 8,552 4 View

Problems with chitin-binding domain fusion protein NOT sticking to chitin resin?

Using the New England Biolabs IMPACT system, we have expressed our protein of interest with a C-terminal fusion of a chitin binding domain (vector is pTXB1 from the NEB IMPACT system). The...

09 October 2019 9,350 3 View

Problems with chitin-binding domain fusion protein NOT sticking to chitin resin?

Using the New England Biolabs IMPACT system, we have expressed our protein of interest with a C-terminal fusion of a chitin binding domain (vector is pTXB1 from the NEB IMPACT system). The...

01 October 2019 7,261 3 View