After deactylation of chitin from snail shells, i have washed the filter residues so many times with hot DW, but it is still strongly alkaline. Can I use dilute HCl in washing and what Molarity is best?
Dear Oluwabukola,
An acid wash can lower the pH of a chitin deacetylation solution, but it is essential to use caution and follow proper safety protocols when handling strong acids. Dilute hydrochloric acid (HCl) is a commonly used acid for this purpose and can be added to the washing solution to lower the pH.
The molarity of the acid solution will depend on the desired pH and the initial pH of the deacetylation solution. A dilute solution of HCl (around 0.1 M) can be used initially, and the pH can be monitored until the desired level is reached.
It is essential to keep in mind that the pH should be lowered slowly and carefully to avoid damaging the chitin fibers. It is also recommended to consult with an expert in this field, as it is best to know the ideal pH range for the chitin deacetylation process and the properties of the chitin you're using.
Additionally, it is essential to use proper personal protective equipment (PPE) when handling solid acids, including gloves, goggles, and a lab coat. And also, it's essential to follow proper disposal methods for the acid solution after use.
I hope I was able to answer your question.
Yours sincerely,
Edgar M Cambaza
Edgar Manuel Cambaza, Thank you for your answer, it was really helpful
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