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I expressed a GFP protein with my protein in Agrobacteria, I guess this protein will localization in the outer membrane, but the results are very confusing, does any friend know where the GFP...
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Hi, I found a plasmid have pSa ori and colE1 ori. Does any scientist know how this kind of plasmid replication in E.coil? If it will make a mistake during replication? Thanks for your kind help.
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I build a big plasmid, more than 17kb， the sequence results are right, but the restriction enzyme digestion has some problems? single enzyme digestion should contain one band, but the results show...
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I would like to do the natural transformation of BT-1 strain, the literature said I can use 50 ul chitin (1mM), does any expert know how to make a chitin solution. because chitin is too stable to...
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I made a new plasmid by gateway cloning, and got the positive colonies by restriction enzyme digestion successfully. but then I transformed into Agrobacteria EHA105, when confirming transform back...
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When I want to transform a plasmid into three Xanthomonas translucens pv. undulosa, found there is one WT resistance to Ampicillin, I tried purify plasmid and transform into E.coil, but I got...
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Is there any method to observe and confirm the protein localization on the bacterial cell membrane?
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