I am trying to stabilize a membrane protein in the bacteria with DMSO and PBA. Wich others chemical chaperones presents good results in order to help the correct protein fold? The chaperone must be able to penetrate the cell membrane since my protein of interest remains inside the bacteria. Thank you !
Andrei Rogoza
Trimethylamine oxide Me3N->O (TMAO) is right compound in vivo;
(CF3)2CO • 3H2O hexafluoroacetone trihydrate is suitable for in vitro applications
http://pubs.acs.org/doi/abs/10.1021/jp207289b
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Niraj Rajesh Bhatt
TMAO has been shown to stabilise and help mutant proteins fold better inside the cells.
I have attached the paper for your reference.
Article Chemical chaperones assist intracellular folding to buffer m...
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