A Vacancy of Sr Scientist
Duration: 12 months
Location: Bethesda, MD/USA
Job Description:
Contribute to the generation of stable cell lines for vaccine and therapeutic protein candidates including monoclonal antibodies, recombinant proteins, and virus-like particles.
Independently execute each step of the cell line development process including plasmid production, transfection, single cell cloning, clone expansion in static culture plates and shake flasks, fed-batch analysis, and clone stability studies.
Use automated platforms for clone selection and screening.
Prepare plasmid DNA using standard molecular cloning techniques.
Participate in cell line development platform design, optimization, and cycle-time reduction studies including methods for clone selection, expansion, ranking, and characterization.
Perform small-scale process development studies using various culture vessels including tissue culture plates, shake flasks, and small-scale bioreactors.
Generate stable pools and perform transient protein production for supply of reagent-grade material.
Provide laboratory support for medium preparation and ordering of supplies.
Perform maintenance of cell counters and bioprocess analyzers, coordination of service visits, and regular ordering of consumables.
Train other laboratory personnel on use of equipment and corresponding processes.
Write and review technical reports documenting process development studies and stable cell line generation work.
Work with other team members to generate experimental plans for process development studies.
Write and review standard operating procedures for cell line development processes and instrumentation.
Maintain complete documentation records, including a detailed and up-to-date laboratory notebook, to support regulatory filings.
Regularly communicate progress to team lead and present work to cell line development team .
Experience Required
B.S. in Biological Sciences with a minimum of 5 years of relevant work experience or M.S. in Biological Sciences with independent research experience in an academic or industrial setting.
Hands-on experience with aseptic technique and mammalian cell culture along with supporting technologies for cell line engineering including cell counting, high-throughput transfection, single-cell cloning, and flow cytometry.
Experience in preparing technical reports, standard operating procedures, and data presentations.
Ability to work independently to execute defined experiments and troubleshoot issues as needed to meet project timelines.
Ability to effectively communicate plans, results, challenges, and ideas to other team members in oral and written forms.
Familiarity with computer software for word processing and data analysis.
Experience in monoclonal antibody and/or vaccine production.
Kindly send me your resume in Word format
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If you know anyone who would be interested, please, let me know.
I'm writing to express my interest in the position you've posted. Please check your inbox for my application.
Thank you Mr. Hossen.
I already sent your CV to the recruiter.
I wish you best luck.
Pedro
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