Can anyone suggest a cheap and the most suitable DNA purification method or kit?
1. Depends from which organism you are isolating from ?
-Bacteria, Eukaryotic cells form culture, plants or fungi ?
2. Depends what your downstream application is going to be ?
IN CASE OF BACTERIA
- If you want to do a PCR, then you might just crack open the cells at 95 degrees for 5 mins, put it on ice for next 5 mins and spin at high speeds to collect the supernatent. The supernatent can be used as a template. This would be the cheapest of all.
-If you want it for any other application, where you require greater amounts of DNA you then, you might have to use a protocol similar to what is given in Molecular Cloning, Sambrook et al. CSH press. Use of Lysozyme, detergent and Ethanol precipitation.
So, please clarify our requirements more profoundly.
- Sudip
the CTAB method is the most common and can say cheap method for DNA extraction
Purification? Genomic DNA? PCR product? Other? If you mean PCR product purification for sequencing, Ethanol precipitation is the cheaper option, but Exo-SAP purification is the best value for money method: a little bit more expensive than Ethanol, but better quality.
I know the cheapest and least laborious method for extracting DNA from blood. It needs only TE, SDS, Proteinase K, NaCl and ethanol. The yield and DNA quality is the same as with extraction by Phenol-Chloroform method. I can write it here if you want to extract DNA from blood.
Ctab is good for plants but i think phenol chloroform is the best one and cheaper
you go through my paper A simple modified method of DNA extraction from seeds for PCR amplifications. Indian Journal of Agricultural Sciences 01/2012; 82(1):75-77, it will help you.
Cheap!! I have once used Ethanol precipitation ....it works pretty well....will send you the full protocol if you want.
depends on the quality of the DNA you want at the end. if it's only for a transformation or a simple restriction digestion then do the ethanol, otherwise use the kit.
Thank you all for your comments and suggestions. Actually I am isolating DNA from ray fish fin. But due to high mucilaginous content and some other factors, band is coming at the top but with contamination at the bottom of the lane. So I want to purify the obtained band for PCR amplification.
The cheapest method is to use plastic disposable columns (I think epoch company have them), loaded with the whatmann GF/F borosilicate glass fiber paper and self-made buffers. You can get a description of this approach in this article: http://www.vitis-vea.de/admin/volltext/W0%2011%20790.pdf
Dear Suni Ge,
Please use DNA investigator Kit -Qiagen which is promising Kit for dry specimens.This might be delayed reply but useful for many dry specimens.
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