N.Subash Chandra Bose Raju

10 Questions 17 Answers 0 Followers

Questions related from N.Subash Chandra Bose Raju

Can you please suggest data base for IR spectrum for polymers ( microplastics)?

I am looking for IR spectrum for various polymers for analyzing micro plastics. That will be really helpful to suggest database or IR spectrum details for various polymers for me. I collected...

19 October 2017 3,941 13 View

Can Someone please tell how to analyze microbial strain difference, polymorphism using 16srRNA?

I have carried out study of insect gut diversity using 16srRNA. We got almost sequences of approximately 50 strains and found out in which seven group of distinct genera formed and gut...

22 July 2015 3,339 3 View

Can you please assist me on how to make rarefaction curve and analysis for microbial 16sr RNA ?

We identified 50 isolates from insect gut and found out through 16sr RNA sequencing. We have found seven groups such as Bacillus sps, Pseudomonas sps and remaining five groups along with other...

04 June 2015 1,964 3 View

Which would be the best outgroup for the following bacterial strains?

I would like to construct a tree on bacterial isolates (16srna) which it has Bacillus sps, Devosia sps, Serratia sps, sps, stenotrophomonas sp, Pseudomonas sps. I constructed a tree based on NJ...

22 April 2015 1,872 7 View

Is there any report on Leptothrips found in Ber?

a few days back, I identified leptothrips from plant Ber. However, most of the thrips are host specific but I found poor reports on this aspect. Leptothrips and Ber . if anyone could have this...

05 January 2015 6,354 1 View

How much molecular difference is required (for mtCoI region) to show different species?

In leafhopper, we have found two new species and verified with taxonomists. To support this view, I did mtCOI amplification and the sequences deposited in GenBank. But in pairwise distance...

08 September 2014 9,558 5 View

Can someone tell me which is good vector for metagenomics study?

I am looking for a good vector for the cloning of gut-micflora metagenome. The source of metagenome is insect gut. I isolated a number of cultures and did 16sRna sequencing. On the other side we...

27 January 2014 9,731 5 View

How do you analyze 16srna sequence using NCBI ?

I received a bacterial colony's forward and reverse sequences for 16sRNA gene after my PCR . I want to analyze the sequences using some good software. How should I analyze these sequences? I am...

03 December 2013 1,426 0 View

Can someone help with a restriction enzyme digestion problem?

I digested pcr products by MboI enzyme, expected 2 bands one at 296 and another at 72bp. I got respectively in few samples which means they are resistant, the wild type will not show any cut...

20 November 2013 4,766 5 View

Ruthraksha Seed dormancy

How long can a Rudraksha seed remain viable? It is often claimed that Rudraksha seeds can live for over 100 years, but is there any scientific evidence supporting this claim? Additionally, if I...

01 January 1970 9,371 4 View