Dear everyone, I always wonder about the effect of concentration change of solvent after it is used to dissolve solute, let's say I use 1%BSA to dissolve pure human serum to make 10% of human serum in BSA. I'm sure that concentration of BSA will be lesser after mixing with human serum and I wonder whether this change in BSA concentration may alter the result of the experiment?
Yes, you are right. It will affect the BSA concentration in the final working serum sample. The dilution factor is an important issue. But from your details, it not clear that what exactly you are looking for? Depending on the experimental target, the dilution of BSA matters under the conditions whether you are looking for the experiment of biology/chemistry or technology development. Good luck
If you know what final concentration of BSA you want in the solution, you can account for the dilution by starting with a more concentrated BSA solution.
However, in this case, since serum protein is mostly albumin, you are diluting the BSA with human serum albumin, so the total albumin concentration will include contributions from both BSA and HSA. It might make more sense to dilute the serum with HSA rather than BSA, if you are trying to maintain the albumin concentration at a certain level.
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