Wongsakorn Muangsom

5 Questions 9 Answers 0 Followers

Questions related from Wongsakorn Muangsom

What could be the reason for the low signal (from high concentration of serum) in indirect ELISA?

Dear, I'm developing an in-house indirect ELISA to detect the present of antibodies in the serum against target protein of the pathogen. The setting of my ELISA is that I have the target protein...

04 September 2023 4,281 7 View

Asking for advice on how to analyse data that is violate assumption of two- way repeat-measured ANOVA?

Dear, Im having trouble with analyzing data using two-way repeat-measured ANOVA. The reason that I use this technique is that my experiment measure cell survival of the the treated cell with...

16 November 2022 3,974 7 View

How many up conformation of RBD on trimeric spike of SARS-CoV-2 is(are) required for successive attachment?

Dear all, I'm trying to find literature to answer my question about the number of required up conformation of RBD within one SARS-CoV-2 trimeric spike protein for successive attachment. Most of...

02 January 2022 7,170 3 View

Change in concentration of solvent when it is used to dissolve solute?

Dear everyone, I always wonder about the effect of concentration change of solvent after it is used to dissolve solute, let's say I use 1%BSA to dissolve pure human serum to make 10% of human...

11 October 2021 8,281 3 View

I wonder about coculturing between A549( in DMEM) and Raji (in RPMI). Do I have to adjust the A549 or Raji to grow in media from different cell line?

I would like to ask whether should I change the A549 to grow in RPMI (and vice versa) for the coculture between A549 (which is growth in DMEM) and Raji ( which is growth in RPMI) for 12 hours or...

24 May 2017 1,517 3 View