Wongsakorn Muangsom

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Questions related from Wongsakorn Muangsom

What could be the reason for the low signal (from high concentration of serum) in indirect ELISA?

Dear, I'm developing an in-house indirect ELISA to detect the present of antibodies in the serum against target protein of the pathogen. The setting of my ELISA is that I have the target protein...

04 September 2023 4,332 7 View

Asking for advice on how to analyse data that is violate assumption of two- way repeat-measured ANOVA?

Dear, Im having trouble with analyzing data using two-way repeat-measured ANOVA. The reason that I use this technique is that my experiment measure cell survival of the the treated cell with...

16 November 2022 4,010 7 View

How many up conformation of RBD on trimeric spike of SARS-CoV-2 is(are) required for successive attachment?

Dear all, I'm trying to find literature to answer my question about the number of required up conformation of RBD within one SARS-CoV-2 trimeric spike protein for successive attachment. Most of...

02 January 2022 7,217 3 View

How to generate humidity during paraformaldehyde fumigation?

Dear all, I'm about to perform fumigation by using paraformaldehyde to react with potassium permanganate. In the procedure, it is stated that 60%-80% of humidity should be reached in order for...

29 November 2021 371 0 View

Change in concentration of solvent when it is used to dissolve solute?

Dear everyone, I always wonder about the effect of concentration change of solvent after it is used to dissolve solute, let's say I use 1%BSA to dissolve pure human serum to make 10% of human...

11 October 2021 8,314 3 View

I wonder about coculturing between A549( in DMEM) and Raji (in RPMI). Do I have to adjust the A549 or Raji to grow in media from different cell line?

I would like to ask whether should I change the A549 to grow in RPMI (and vice versa) for the coculture between A549 (which is growth in DMEM) and Raji ( which is growth in RPMI) for 12 hours or...

24 May 2017 1,551 3 View