I would like to ask whether should I change the A549 to grow in RPMI (and vice versa) for the coculture between A549 (which is growth in DMEM) and Raji ( which is growth in RPMI) for 12 hours or not. Will the different type of media interfere the characteristic of another cell line in coculturing? i tried to adjust the A549 to grow in RPMI by gradually add more percent of RPMI to the culture media. But at the 100% RPMI, it seemed that the A549 cell got stress and the stress maintained for the later passages :(
Agnieszka Bojko
You must check the amount of sodium pyruvate in RMPI medium, because A549 cells are very sensitive to the low level of it. If there is less sodium pyruvate in RPMI than in the DMEM medium, add the missing amount of that compund.
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