Cells not growing in large scale protein expression?

More Fiazall Tufail's questions See All

What should we do if?

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I want some literature regarding Phosphate nanoparticles, their synthesis and mainly about their transport in porous media?

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

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Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

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What urea buffer can i use for biotagged protein extraction from streptavidin beads?

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Jorgaq Pata

Do you use any antibiotics and at what concentration? How much of your preculture are you putting in your 1L medium?

Michael J. Benedik

Do your overnight cultures look normal and not have any debris? In addition to ensuring you have the right antibiotic concentration I wonder whether you might have phage contamination.

Manuele Martinelli

Dear Fiazall

it is quite strange:

1 liter in which flask?

It do not growth or it stop to growth at certain OD?

best regards

Manuele