Cells not growing in large scale protein expression?

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Jorgaq Pata

Do you use any antibiotics and at what concentration? How much of your preculture are you putting in your 1L medium?

Michael J. Benedik

Do your overnight cultures look normal and not have any debris? In addition to ensuring you have the right antibiotic concentration I wonder whether you might have phage contamination.

Manuele Martinelli

Dear Fiazall

it is quite strange:

1 liter in which flask?

It do not growth or it stop to growth at certain OD?

best regards

Manuele