Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

What should we do if?

if we do not get appropriate GC content and melting temperature while designing primers for full gene amplification? i have a nucleotide sequence of sars cov2 surface glyco-protein of which i have...

20 July 2020 8,085 2 View

What should be the value for dissipated energy factor for the varification of the experminetal data for a concrete beam?

What should be the value for dissipated energy factor for the varification of the experminetal data for a concrete beam? What exactly should i choose from the experimental data to numerical...

16 October 2019 4,417 2 View

Can any one share a reliable transfer function model of wind and/or solar energy generation sources for load frequency control (secondary control)?

We have transfer functions for other generation sources like non-reheat steam turbine (1/1+st). I am looking for same kind of transfer function for Renewable sources.

21 November 2018 7,457 3 View

How to stabilize the pH of NaNO3 solution?

I am doing a soil column experiment, using quartz sand as a transport medium. We are using 0.001 M NaNO3 as a background solution. Before injection to soil column the pH was adjusted to 7.5 with...

12 August 2018 9,630 2 View

Power Control With Z-Source Converter Based Unified Power Flow Controller UPFC?

I want to simulate on MATLAB SIMULINK Three Phase Z source based UPFC for IEEE-30 Bus bar standard with line to line ground fault. any one done work on ZSI UPFC , kindly guide . regards

06 April 2018 3,504 1 View

I want some literature regarding Phosphate nanoparticles, their synthesis and mainly about their transport in porous media?

Phosphate nanoparticles

05 February 2018 4,382 3 View

Anyone can suggest me an effective quantitative instrument for assessing the performance of software packages among oil and gas industries?

28 May 2016 1,570 5 View

How can I compare crash characteristics between two locations?

I have to compare the crash characteristics two roads at two different geographical conditions, A and B. A have their own segments A1,A2,A3 and A4. B also have their own segments B1,B2,B3,B4 and...

08 December 2014 382 22 View

Any published article on this work pls?

I want to see how it will help in optimization of a harvesting machine

01 January 1970 8,436 2 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

The most suitable method to calculate the stress intensity factor in the presence of contact between the crack lips?

I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...

21 February 2021 8,694 4 View

Any reference to learn numerical methods for Viscoelastic compressible fluids(oil) (CFD)?

I am developing a solver to simulate compression of oil in shock absorber filled with viscoelastic compressible oil. The model will consider the loss due to viscosity which will decide the...

15 January 2021 1,707 1 View

How to implement truss element with no effect of compression in ansys workbench static structural?

I was trying to add ligaments in cervical spine geometry. There it is mentioned in the existing literature to add the ligaments in the geometry as truss element with no effect of compression....

03 January 2021 2,205 0 View

Why does bending stress(Sx) in ANSYS APDL direct below of point load FE 2D modelling simply supported point load higher than exact solution in value?

Why does normal bending stress/flexural stress value (Sx) just direct below a concentrated load of simply supported beam which is compression stress higher than the hand calculation Exact solution...

03 January 2021 8,462 3 View

Is the concrete tensile stress-strain curve a tenth of its compressive one ?

The known compressive stress-strain curve of concrete corresponds to the negative strains despite the selected axis shift to the positive strains values. The concrete tensile yield stress is known...

27 December 2020 7,309 7 View

What happens to a gas after compressing it for 10 years?

Will it lose a fraction of its expansion power assuming it was insulated in this system? Because I am designing an insulated gas spring. what I want to know if the gas will lose some of its...

07 December 2020 6,150 3 View

How to apply the Orthogonal Matching Pursuit(OMP)Algorithm on images?

Hello to all, I am research scholar and pursuing Ph.D in the field of Compressive Sensing. I want to know , how to apply the OMP algo. for reconstructing the compressed image. Since i have done...

06 December 2020 8,012 6 View

Assessing quality of CPR?

In simulation practice/training, are there set standards for monitoring quality of a resuscitation attempt? beyond rate/depth of compressions and time off chest?

23 November 2020 5,182 12 View