Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

What should we do if?

if we do not get appropriate GC content and melting temperature while designing primers for full gene amplification? i have a nucleotide sequence of sars cov2 surface glyco-protein of which i have...

20 July 2020 8,085 2 View

What should be the value for dissipated energy factor for the varification of the experminetal data for a concrete beam?

What should be the value for dissipated energy factor for the varification of the experminetal data for a concrete beam? What exactly should i choose from the experimental data to numerical...

16 October 2019 4,417 2 View

Can any one share a reliable transfer function model of wind and/or solar energy generation sources for load frequency control (secondary control)?

We have transfer functions for other generation sources like non-reheat steam turbine (1/1+st). I am looking for same kind of transfer function for Renewable sources.

21 November 2018 7,457 3 View

How to stabilize the pH of NaNO3 solution?

I am doing a soil column experiment, using quartz sand as a transport medium. We are using 0.001 M NaNO3 as a background solution. Before injection to soil column the pH was adjusted to 7.5 with...

12 August 2018 9,630 2 View

Power Control With Z-Source Converter Based Unified Power Flow Controller UPFC?

I want to simulate on MATLAB SIMULINK Three Phase Z source based UPFC for IEEE-30 Bus bar standard with line to line ground fault. any one done work on ZSI UPFC , kindly guide . regards

06 April 2018 3,504 1 View

I want some literature regarding Phosphate nanoparticles, their synthesis and mainly about their transport in porous media?

Phosphate nanoparticles

05 February 2018 4,382 3 View

Anyone can suggest me an effective quantitative instrument for assessing the performance of software packages among oil and gas industries?

28 May 2016 1,570 5 View

How can I compare crash characteristics between two locations?

I have to compare the crash characteristics two roads at two different geographical conditions, A and B. A have their own segments A1,A2,A3 and A4. B also have their own segments B1,B2,B3,B4 and...

08 December 2014 382 22 View

Any published article on this work pls?

I want to see how it will help in optimization of a harvesting machine

01 January 1970 8,436 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View