Hello, I am isolating PBMCs to obtain Endothelial Progenitor Cells, but during the culture, I discovered that both of my plates included "unknown creatures" 3 days after the isolation; may I know what these are?
To me it looks like a bacterium contamination that would have come from any of the reagents used for/during culturing, like culture medium, serum, trypsin, freezing stocks etc., Now you can doubt all the reagents. So, now you try testing each one those individually to check where the contamination came from or you better start over fresh with all new. Also try to check freeze stocks from different dates. Make sure the water baths are maintained clean. Most importantly, handling techniques will be the culprit in most cases !
They look like dead cells to me. Cells tend to die once they exceed their passaging period. And dead cells will float along with the media. Wash with 1XPBS would remove them.
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