How to calculate visual angle of a visual stimulus?

I am currently trying to figure out how to calculate the visual angle of some visual stimuli I used in an experiment. I think I have all of the information I need to calculate this. One is a...

15 February 2021 3,189 2 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

How to make sure self-ligation of a linear plasmid?

I want to ligate a linear 1.7kbp plasmid with the EcoRI sites at two sides, but when I use the quick ligase kit from NEB to test the ligation efficiency it is hard to ligate it. Is there anyone...

08 December 2020 3,442 3 View

What effect does light have on the SLS plant tissue decelluarization process?

does it speed it up?

29 November 2020 6,168 3 View

Significant degradation of RNA in RNAlater storage at 4C for longer than a month?

I misread the amount of time that RNA was stable in RNA later at 4 C as several months instead of a month. I have e zebrafish samples that have taken me a 2 months to collect and they have been...

23 October 2020 8,085 3 View

How to present the results of a binary logistic regression for an ecological paper?

I conducted a binary logistic regression using the glm function in R. My binary predictor is the presence or absence of my study species. I have two significant variables but am just not sure what...

19 October 2020 5,398 3 View

Basic question - how to make a cell dissociation buffer?

Dear All, I'm looking to make a cell dissociation buffer from lyophilised collagen I (Gibco, 12648010). The protocols I've been looking at all specify concentrations of enzymes in terms of...

06 October 2020 506 3 View

What is the best way to perform a serial dilution of E.coli, when the culture is starting on a solid media (LB)?

I have an LB growth media plate with E.coli on it. I want to dilute the culture. I am working with 3M Coliform detection plates, and I want to get a solution of E.coli diluted enough to grow...

27 August 2020 3,043 3 View

Why fluorescence signal drops during thermal shift assay?

I am performing a TSA to investigate binding of a ligand to the protein by performing a serial dilution of ligand against same conc. of protein (~2uM). Interestingly at low ligand conc the...

02 August 2020 3,167 1 View

Writing specific contact surface behaviour using Userinter.F in ANSYS?

Hello, Is there anybody who has experience developing any user subroutine using Userinter.F for contact simulations in ANSYS? Any advise and example would be appreciated highly. thanks a lot

21 February 2020 6,495 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View