How to isolate proteins solubilized in ethanol?

hi, i'm conducting a study on wheat. I extracted the gliadins from these cereals and now they are solubilized in 60% ethanol. Is there a method of eliminating ethanol (other than boiling) that...

25 October 2020 8,506 3 View

How can I transfer a small molecule from a surface into a cell?

I am dealing with support-mediated drug delivery systems, and in particular I have some cancer cells adherent on a collagen surface. I loaded a hydrophilic drug on collagen by physisorption, then...

03 August 2019 233 4 View

How to control the API content on pharmaceutical liquid inside bags by NIR spectroscopy?

25 January 2017 905 3 View

Can Soluble solide content be measured by hyperspectral system?

06 June 2016 1,049 2 View

Is anyone looking for collaborators in theoretical physics?

Dear Research Community, I am very interested in offering my mathematical and especially numerical analysis expertise to solving problems involving the Einstein Field Equations, with application...

06 July 2014 6,525 7 View

What would happen if some paper is published in a journal and the main idea is extracted from some previous paper without proper referencing?

What would happen if some paper is published in a main journal and the main idea is extracted from some other paper originally and previously published only online or on some secondary...

01 January 1970 7,882 23 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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02 March 2021 3,060 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View