Cell lysis with Triton X-100 ?

Vittorio Monticelli @Vittorio-Monticelli

10 August 2022 0 2K Report

-does Triton X-100 also permeabilize nuclear membranes?

-what is the ideal concentration of Triton X-100 to use?

-do low temperatures slow down or prevent cell lysis (by Triton)?

Badges
Science topic

More Vittorio Monticelli's questions See All

How to isolate proteins solubilized in ethanol?

hi, i'm conducting a study on wheat. I extracted the gliadins from these cereals and now they are solubilized in 60% ethanol. Is there a method of eliminating ethanol (other than boiling) that...

25 October 2020 8,675 3 View

What is the explicit of vertical epicyclic frequency in Schwarzschild and Kerr spacetime outside of the equatorial plane?

I am interested in studying the vertical epicyclic frequencies in Schwarzschild and Kerr metrics in the whole 3D space, not only in the equatorial plane. I have looked up in the literature and I...

11 March 2020 380 1 View

How can I transfer a small molecule from a surface into a cell?

I am dealing with support-mediated drug delivery systems, and in particular I have some cancer cells adherent on a collagen surface. I loaded a hydrophilic drug on collagen by physisorption, then...

03 August 2019 326 4 View

How much could be the cost of an agrarian lot if we count its ecological services?

Following the principles of Landscape Bionomics, we can find the equivalence between bionomics costs and economic costs when we have to introduce a new inhabitant in a given region: the result of...

24 March 2019 904 1 View

Anyone can help with this bone?

Together with my colleague Gerlando Vita I'm working on late Paleolithic sediments from San Teodoro cave (NE Sicily). There we found a mystery, small bone. In places (see the withish side in fig....

23 October 2018 5,181 3 View

How to control the API content on pharmaceutical liquid inside bags by NIR spectroscopy?

I would like to try to control the content or concentration of active principle in a liquid mixture inside a plastic infusion bag. At the same time i would like to monitor if the API is what we...

25 January 2017 1,012 3 View

How long continue Aspirin prophylaxis after a surgical mitral repair with use of artificial cords and annuloplasty ring, with LVEF of 50%?

Is there literature-guidelines in this field?

29 June 2016 3,824 14 View

Can Soluble solide content be measured by hyperspectral system?

Has anybody experience on the measurements of Soluble solide content (SSC) and °BRIX in tomatoes or other vegetables by the use of hyperspectral camera? In the case, how it is possible to made...

06 June 2016 1,215 2 View

Could you please help me find the Rindler metric in spherical coordinates?

If the particle accelerates radially, is the metric the same as in cartesian coordinates?

11 July 2014 2,513 5 View

Is anyone looking for collaborators in theoretical physics?

Dear Research Community, I am very interested in offering my mathematical and especially numerical analysis expertise to solving problems involving the Einstein Field Equations, with application...

06 July 2014 6,624 7 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

I need JCPDS file of LSFCO nanomaterial. Can anyone provide me?

Require for Rietveld refinement in XRD.

08 August 2024 3,081 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View