How to isolate proteins solubilized in ethanol?

hi, i'm conducting a study on wheat. I extracted the gliadins from these cereals and now they are solubilized in 60% ethanol. Is there a method of eliminating ethanol (other than boiling) that...

25 October 2020 8,506 3 View

How can I transfer a small molecule from a surface into a cell?

I am dealing with support-mediated drug delivery systems, and in particular I have some cancer cells adherent on a collagen surface. I loaded a hydrophilic drug on collagen by physisorption, then...

03 August 2019 233 4 View

How to control the API content on pharmaceutical liquid inside bags by NIR spectroscopy?

25 January 2017 905 3 View

Can Soluble solide content be measured by hyperspectral system?

06 June 2016 1,049 2 View

Is anyone looking for collaborators in theoretical physics?

Dear Research Community, I am very interested in offering my mathematical and especially numerical analysis expertise to solving problems involving the Einstein Field Equations, with application...

06 July 2014 6,525 7 View

What would happen if some paper is published in a journal and the main idea is extracted from some previous paper without proper referencing?

What would happen if some paper is published in a main journal and the main idea is extracted from some other paper originally and previously published only online or on some secondary...

01 January 1970 7,882 23 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

How can I measure the surface interaction or surface interfacial chemistry between polishing abrasives and upper surface of substrate ?

I am working on polishing abrasives. I need to do few characterizations ? which can provide the on time surface chemistry of substrate with polishing abrasive during optical polishing. I would...

18 February 2021 5,319 3 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

Inquiry regarding engineering journals for free publications for my dissertation (laser polishing of 3D printed parts?

my dissertation was on laser polishing of 3D printed plastic parts, i want to publish it, can anyone suggest me a free copy engineer journal for this purpos, plz?

01 February 2021 7,140 4 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View

How to gate mouse MDSC cells through flow cytometry?

On inducing the mouse bone marrow cells with X compound, there is a change in cells morphology and characteristics. In that case how to gate these cells, as these cells are showing suppressive...

27 January 2021 608 2 View

How to polish the samples which are very prone to oxidation for metallography?

I am trying to polish the rare earth based sample for SEM analysis. But the sample is getting oxidized immediately . Can anyone suggest a way to polish these kind of samples?

27 January 2021 4,699 2 View

How much of cells needed to get good number of RNA ?

Hello I am growing MSCs cells in either 24 wells or 12 wells plate and then I need to lysate these cells for RNA extraction I ususally get RNA from 5 to 30s ng/ul Is there is any idea or tips...

26 January 2021 8,531 3 View

How to do rat bone Immunofluorescence, Please suggest?

Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo

26 January 2021 4,165 3 View