Can features from a feature selection algorithm based on classification be used for regression?

I am working on a regression task where I am trying to predict future values of a stock/resource. At the moment, my model uses a large set of lags as input features and I want to use feature...

31 August 2020 2,409 3 View

Has SARS-CoV2 been isolated, purified and demonstrated to be the cause of COVID19?

Pondering the riddles of life, I was wondering whether the virus SARS-CoV2 has been isolated, purified and demonstrated to be the cause of COVID19? Is there a publications which shows this? I can...

07 June 2020 7,414 46 View

What books and electronic resources would you recommend for introducing research and research philosophy and methodology?

If you had to train a researcher and you could only do so by prescribing them books and other written and electronic sources, which resources would you use most confidently? Also, think in terms...

15 May 2020 1,630 7 View

A typical spectrum of soundwave in a lab environment?

Hello all, I was wondering if there exists some general trend of the soundwave's spectrum. Something similar like the decaying behavior of ground vibrations. I'm especially interested in the...

30 March 2020 3,286 3 View

NEB HiFi Assembly started failing on all constructs, competent cells are fine. How do I solve this?

I am trying to assemble multiple constructs: - 2 fragments (1300bp and 2000bp) into a 3.3 kb vector - 1 fragment (2000bp) into 5 kb vector - another 2000bp fragment into the same vector - 1...

19 February 2019 1,849 11 View

Why is my NTO (Niobium-doped Titanium Oxide) not conductive?

I have sputtered NTO from an alloy target consisting for 99 at. % of titanium and 1 % of niobium. It was reactive DC magnetron sputtering, with a mixed atmosphere of oxygen and argon. The...

12 October 2016 8,740 16 View

Anyone familiar with an antibody to stain microglia in zebrafish?

I was wondering if anyone knows an antibody that can be used for staining microglia in zebrafish? I am familiar with 4c4 and L-plastin antibodies, but these are not perfect for me. Is anyone...

14 December 2015 7,427 9 View

When a dried blood spot is prepared (EDTA whole blood), will all cells be completely lysed when dry? Or does drying not necessarily disrupt the cells?

When blood is eluted from the filter paper with phosphate buffered saline, would you expect that cellular material is still contained in the cells or would you assume that cellular material will...

17 March 2015 7,859 7 View

Can anyone help with decomposition of primes in maximal real cyclotomic subfield?

I am looking for a reference giving the decomposition of a prime p in the maximal real subfield of a cyclotomic number field Q(zeta_m). Something along these lines: If p is a prime not dividing m,...

10 April 2014 9,140 1 View

What is the best method to compare the evolution of a single pathway within different species with the evolution of the entire genomes?

For a certain bacterial pathway I conducted some evolutionary analyses. Now, I would like to compare this evolutionary model with the evolutionary model of the entire genomes. How can I do this?...

02 March 2014 378 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View