Cell culture 3T3-L1 adipocytes. I can grown in DMEM medium with or without pyruvate pyruvate?

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Alessio Reggio

Hi, Orlando.

Usually the growth medium for 3T3-L1 is composed by DMEM F-12+ 1%P/S.

Sibtain Afzal

Hi Orlando

Although there are several preadipocyte cell lines reported, I am going to send you a protocol from a book that describe mainly the 3T3-L1 cells, which are best characterized and widely used for molecular biological studies.

I hope it will help you out

Best Regards

Dear Orlando

here is anotherbook which might be very useful for you. its a gift for u.

best regards