Hello,
I'm experiencing some problems trying to monitorate my culture growth. Usually, in my lab people do that using the espectrophotometer, but I can't use it because my experiments do not allow cells to be exposed to light in a specific wavelenght, only white light.
Then I started to count cells in photos taken by videomicroscopy, but i find it too weak in accuracy, because the cells have not a simple shape, they´re like fibroblasts, and I can´t use trypsin to make them round. Does anybody knows any software that makes the image analysis that I need to tell how much my culture is expanding more accurately? Could be something that makes the analysis of image "density", not necessarily a cell counter. Today I'm exploring the software ImageJ, but I'm still uncertain if it's texture analysis is really my best option.
Best regards,
Elga.
I think this situation can not be automated and your own eyes is the best software. However, if the problem is that the cells form small aggregates and if your cell are motile, perhaps time-lapse videomicroscopy can help.
Something like that:
Wick, N., S. Thurner, et al. (2003). "Quantitative measurement of cell migration using time-lapse videomicroscopy and non-linear system analysis." Histochemistry and Cell Biology 119(1): 15-20.
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