Has anybody used a CD13 antibody for labelling pericytes in mouse brain tissue? The antibody cannot be raised in Goat due to a second antibody hosted in this species.
Here are a few references to CD13 antibodies used for labeling pericytes in mouse brain tissue:
In this study, the authors used a mouse monoclonal anti-CD13 antibody (clone WM15, Abcam) for labeling pericytes in mouse brain tissue.
2. Feng J, et al. "Targeting Pericyte Function in Brain Vascular Diseases." Frontiers in Aging Neuroscience (2015).
In this review, the authors mention that several anti-CD13 antibodies have been used for pericyte labeling, including mouse monoclonal antibodies (clone WM15, Abcam) and rabbit polyclonal antibodies (LSBio).
3. Fichtner I, et al. "Pericyte loss accelerates blood-brain barrier dysfunction during aging." Scientific Reports (2017).
In this study, the authors used a mouse monoclonal anti-CD13 antibody (clone WM15, Abcam) for pericyte labeling.
The commercial suppliers of mAb WM15 (besides Abcam, it is offered by LSBio, St John's Lab, et al.) consider it human-reactive. A couple of other mAbs that you might want to look at, which are from rat and specifically mouse-reactive, are ER-BMDM1 and R3-63, which are available from several suppliers. Abcam has a couple of user reviews for R3-63:
https://www.abcam.com/cd13-antibody-r3-63-ab33489.html?productwalltab=abreviews&productWallTab=Abreviews
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