I am using X-Bridge C18 column with 25cmx4.6mm,5µ
MPA: 0.02M Ammonium dihydrogen phosphate with pH 2.0 with OPA +0.1mL of TFA in 1000 mL of water
MPB:MPA:ACN(20:80)
Time % of A % of B
0 95 5
5 95 5
8 75 25
25 65 35
33 0 100
45 0 100
45.1 95 5
55 95 5
My main analyte Retention time is 13 mins
and diluent is 100% Methanol
Bruce Neagle
Why did you post this question a 2nd time? Didn't like the answers below and fishing for the right answer?
https://www.researchgate.net/post/I_am_facing_a_problem_about_carryover
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Narong Chamkasem
What is OPA? put the full name. Adding 100% methanol in the samplebis not a good practice, it will distort the peak shape because it is stronger than the initial mobile phase.
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