I have some mouse tissues that have been fixed in 4% PFA, cryoprotected in 30% and embedded in OCT blocks. Do I need an antibody to eYFP?
I'm trying to use autodock vina, and I received the following error for the ligand file when running the configuration: "Parse error on line 26 in file "15.pdbqt": Unknown or inappropriate...
21 October 2020 7,245 1 View
I am looking for data on the implementation of a microbiological reduction step for frozen fruit pieces. I have information on cold plasma/UV but am unable to locate much data on gaseous methods...
24 November 2019 6,131 8 View
This seems to be the only kit (made by Invent Biotechnologies) that's based on a non-density gradient and ultracentrifugation method. Has anyone used it successfully to isolate lysomes from...
20 November 2019 7,210 3 View
10 November 2019 9,907 3 View
My question pertains to human heart tissue previously embedded and frozen in OCT. We have recently cut sections of varying thickness (100-1000 um) for processing and staining while free-floating....
21 October 2019 7,148 3 View
Hi there, I am carrying out a study on factors affecting electric vehicle adoption. I am using a likert scale format (from strongly disagree to strongly agree) for all of the predictor variable...
13 August 2019 258 3 View
My study is on electric vehicles and I want to test whether factors such as cost and range anxiety have significant effects and their degree of influence when analysing the intent to adopt. I am...
03 July 2019 9,547 10 View
I am setting up a fragment analysis assay in which I need to calculate the ratio of the two peaks. The protocol I have inherited suggests using Peak Height, and I see this used a lot generally for...
07 May 2019 9,369 1 View
If you have a validated RNA structure (with or without pseudoknots) can you back calculate a theoretical free energy for that structure? I know many programs that calculate a free energy and try...
20 January 2019 7,763 2 View
Hi, I'm looking to incorporate a formula into a database I'm building in Excel that returns the identification of a chemical based on it's linear retention index (LRI). Some of these chemicals...
26 April 2018 1,779 2 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View