I have some mouse tissues that have been fixed in 4% PFA, cryoprotected in 30% and embedded in OCT blocks. Do I need an antibody to eYFP?
Needed for a dissertation,
17 July 2024 2,004 0 View
Hello, I've just attempted a wet transfer of a western, and had set up the cassette and tank, and run @ 100V and set it to 90 mins. I had pre-chilled the transfer buffer - but didn't put the...
25 June 2024 7,419 2 View
I am writing a research paper on resolving workplace conflict. I need help writing problem statement and proposal and any suggestions for references.
19 March 2024 8,986 3 View
I have been in the camp that believed bison were not part of the natural fauna in the desert grasslands. Individual bones found scattered throughout were as a result of trade among indigenous...
09 December 2023 2,452 1 View
Now lists on a partial title.
02 December 2023 9,661 1 View
I have been attempting to purify a streptagII-tagged protein from mammalian cells using streptavidin-magnetic beads without success. The reason I'm using streptavidin and not streptactin is that...
08 March 2023 367 0 View
I’m Using a water gas shift reaction with a high temperature shift in an adiabatic Packed bed reactor to synthesise ammonia. I’m struggling to find the correct rate equation and therefore volume...
28 February 2022 3,883 3 View
Hi For an assignment I need to critically evaluate social skills interventions and executive functions that are recommended specifically for children between 6 - 12 with FASD but I'm struggling to...
20 February 2022 2,773 1 View
I am doing a project for the final module of my degree with the OU, and looking at the relationship between epistemological beliefs and peoples attitudes towards constructivist online learning...
09 January 2022 5,485 4 View
Working on a research project and we have performed a motility assay on c. elegans with different essential oils. Wondering if there was any way to test if it statistically significant.
28 November 2021 6,383 4 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View