Ainslie Derrick-Roberts

6 Questions 3 Answers 0 Followers

Questions related from Ainslie Derrick-Roberts

Has anyone used the MinuteTM lysosome isolation kit to isolate lysosomes?

This seems to be the only kit (made by Invent Biotechnologies) that's based on a non-density gradient and ultracentrifugation method. Has anyone used it successfully to isolate lysomes from...

11 November 2019 10,100 3 View

What's the best way to quantify keratan sulphate GAG in tissue samples?

We have previously used uronic acid for other GAGs. Any advice?

08 August 2017 7,747 1 View

Does anyone have a way to detect wildtype cre in a Col2a Cre tissue restricted mouse model?

I need a way to distinguish homozygous Col2acre mutants from heterozygous Col2acre after they have been bred with a flox/flox mouse model.

07 July 2016 1,073 2 View

Can you tell me what the best way to detect positive eYFP staining?

I have some mouse tissues that have been fixed in 4% PFA, cryoprotected in 30% and embedded in OCT blocks. Do I need an antibody to eYFP?

02 February 2014 3,328 0 View

Has anyone used PCR reagents exposed to UV?

I had Phusion HF buffer, dNTPs, Q solution and DNA primer stocks (100uM) exposed to UV this morning. Unsure how long the UV was on for. Has anyone had any experience with whether they would be ok...

03 March 2013 6,277 11 View

Has anyone using Phusion DNA polymerase done the initial denaturation at 95 degrees rather than 98 degrees?

Does this make a difference to the outcome of the PCR?

09 September 2012 632 8 View