Anwesha Sarkar

5 Questions 6 Answers 0 Followers

Questions related from Anwesha Sarkar

Can you store sample after elution in an IP at -20 deg?

Hi, I am performing an IP-WB using GFP magnetic agarose beads from chromtek. In the protocol, after washing, they mention that if elution performed by boiling beads with 2X-sds sample buffer and...

16 June 2022 2,783 0 View

What is the mol. weight of GFP-11 tag?

Hi, I am trying to find out the mol. weight of GFP 11 because it is tagged to my protein of interest which is 17kDa. GFP 11 has been tagged with my protein of interest while GFP 1-10 is tagged...

10 December 2020 2,141 1 View

Which is the best stripping method for proteins having molecular weight 90kDa and above?

Hi, I would like to know which is the best stripping method for proteins having mol. wt. around 90 kDa or more. I am using human samples. I used 0.5M EDTA stripping buffer by boiling PVDF...

09 September 2020 5,997 2 View

What is the best solvent for dexamethasone so that it does not get toxic for the cells?

Hi, I am using C2C12 cells. I need to treat them with dexamethasone. I would like to know which is the best solvent to dissolve dexamethasone so that it is not toxic to the cells? I am using dex...

23 June 2020 5,123 6 View

Myosin expression in myotubes?

I would like to know if myosin expression is higher in myotubes? I am using C2C12 cells, and in western blot, I find myosin expression to be more in myotubes.. Why is it so?

01 March 2020 615 3 View