Can you share a mammalian membrane protein extraction protocol?

More Adam P. Fischer's questions See All

Non-parametric version of the wo-way repeated measures ANOVA?

Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...

24 July 2024 3,284 6 View

Phengaris rebeli on Picea abies?

Today I took this video in Southern Bavaria, Germany. Video: https://www.youtube.com/watch?v=cCy1eLM6aE0 A female Maculinea rebeli was continuously tapping the leaves of Picea abies with its...

15 July 2024 8,099 4 View

Please tell me the scientific guidelines (books) on Trombidiformes ticks?

books and scientific papers on the morphology, taxonomy and so on of ticks of the suborder Trombidiformes

17 June 2024 948 2 View

Do plants produce hormones that have the same influence properties of animal hormones?

I know that all animals and insects produces hormones. Do plants produce hormones that can have an equivalent effect on an animal like animal hormones?

13 June 2024 3,860 1 View

Micelle vs liposome?

I hope we will not need to reinvent the wheel. Quick (maybe not very smart) question: How do you distinguish between these tw particles? Any proposed technique will definitely provide the answer....

21 May 2024 9,073 5 View

Why there is a increase in participants in road running events?

There is a huge demand for road running events such as marathons in past couple of years. why?

01 May 2024 3,612 0 View

Which of the following proliferation-tracing dyes perform the best?

I'm designing a cytotoxicity assay to measure the effect of treatment on proliferation. My specific objective is to compare the number of cells with 1 and 2 divisions in treated cultures to...

24 April 2024 2,566 0 View

Cointegration in case of the mixture of I(0) and I(1) stationary variables?

Hi! I want to use the ADL model for my data analysis. However, after performing a stationary test, dependent and 6/8 independent variables are stationary only in differences. The other two are...

23 April 2024 9,017 3 View

"Abaqus/standard analysis exited with an error". Is there a solution?

Hello, I am currently trying to run a simulation for my research using abaqus. I have installed abaqus 2022 version and also followed the required steps to link abaqus with intel fortran. I have...

15 April 2024 3,123 1 View

What is the procedure for implementing the DDMRP methodology on SAP S/4HANA and how to do it?

i'm an intern and this is what i'm suposed to do

15 April 2024 6,048 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Adam B Shapiro

This paper may have some useful techniques for you.

http://www.jbc.org/content/269/5/3745.full.pdf

Steingrimur Stefansson

Hi Adam, you could try triton X-114 phase separation.

http://www.jbc.org/content/256/4/1604.full.pdf+html

Katherine J Vallejos Sánchez

Very useful information. Did you try with any extra method?

Thanks.