Adam P. Fischer

6 Questions 6 Answers 0 Followers

Questions related from Adam P. Fischer

Is it valid to compare qPCR data collected from two different instruments for the same study?

I am beginning a project that will require me run upwards of 1000 individual full qPCR plates to measure the natural variation in gene copy number (mito genes). I have access to two departmental...

03 February 2018 503 1 View

Can anyone recommend excellent drug pathway interaction software?

I am look for some type of software or tool that will allow me to enter a PubChem SID number or other identifiers and then provide me with molecular pathways that may be influenced by said drug. I...

01 November 2017 5,820 1 View

Suggestions for a Drosophila nomenclature handbook?

Does anyone have any suggestions for a Drosophila nomenclature guide or handbook for beginners? I recently started to work with Drosophila however I have no experience and the nomenclature is...

04 October 2017 3,679 6 View

Help with cancer cell spheroid formation and 3D invasion assay?

I have recently began culturing a melanoma cell line in hanging drop plates to form spheroids with the intention of harvesting the spheres into the bottom of a 96-well round bottom plate and...

14 September 2016 325 3 View

Can you share a mammalian membrane protein extraction protocol?

I need to extract membrane transporter proteins from mammalian cell lines. Does anyone have a reliable protocol that would extract and enrich membrane proteins containing several TM domains? I...

15 January 2015 7,483 3 View

Can anybody help me troubleshooting my problem with BD biocoat matrigel invasion chambers?

I have used BD invasion chambers before on WM9 metastatic melanoma cell and they worked well showing lots of invasion for the controls (repeated 3 times). Now using the same cells and methods the...

15 December 2014 1,680 2 View