Did you find one?

Hi Natalie,

I have found a good vector and have managed to overepress RNaseH1 in my cell line. I am going to do a DIP to see if it gets rid of R-loops. So watch this space!

Diu,

I am just starting my R-loops project. I am trying to find a vector to overexpress RNase H, and also ways to monitor R-loops formation. 

Where did you get the vector? Could you please send me some?

Do you use S9.6 antibody? It seems like there might be problems with it: 

http://www.ncbi.nlm.nih.gov/pubmed/25890199

Hi Natalie,

I got the vector from Natalia Gromak at the Dunn school of pathology. You can ask her for some.

Yes, I do use S9.6 antibody and have no problem with it.