I would like to use a colorimetric method for estimation of cellulose in fruit peels?
Look this paper http://www.sciencedirect.com/science/article/pii/S0003269769800096
Is one option
I've read about estimation of cellulose with colorimetric method before. You can use Anthrone method (Hedge and Hofreiter, 1962). This is the standard procedure:
1. Add 3 mL acetic/nitric reagent to a known amount (0.5 g or 1 g) of the sample in a test tube and mix in a vortex mixer.
2. Place the tube in a water bath at 100 degree celcius for 30 min.
3. Cool and then centrifuge the contents for 15-20 min.
4. Discard the supernatant.
5. Wash the residue with distilled water.
6. Add 10 mL of 67% sulphuric acid and allow it to stand for 1 h.
7. Dilute 1 mL of the above solution to 100 mL.
8. To 1 mL of this diluted solution, add 10 mL of anthrone reagent and mix well.
9. Heat the tubes in a boiling water bath for 10 min.
10. Cool and measure the color at 630 nm.
11. Set a blank with anthrone reagent and distilled water.
12. Take 100 mg cellulose in a test tube and proceed from step no. 6 for standard. Instead of just taking 1 mL of the diluted solution (step 7) take a series of volumes (say 0.4 to 2 mL corresponding to 40-200 micro gram of cellulose) and develop the color.
For calculation, draw the standard graph and calculate the amount of cellulose in the sample.
For materials, 1. acetic/nitric reaget: mix 150 mL of 80% acetic acid and 15 mL of concentrated acetic acid, 2. anthrone reagent: dissolve 200 mg anthrone in 100 mL concentrated sulphuric acid. prepare fresh and chill for 2 h before use, 3. 67% sulphuric acid.
I hope it helps
I tinnk that exist on standart Methos manual for chemical Ingenieers a test where you can check if your sample had or not,. iodometric test is an indicator positive or negative if you just to know have it or not
Hello Hazmi YN
Can you please give me the reference of the above method ?
Hi Bharat Bhushan Negi
I've read on Sadasivam, S. & Manickam, A. 1996. Biochemical Methods. New Age International. Page 13 - 14.
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