The MB+-modified aptamer, MB+-(IV), reveals a quasi-reversible redox wave at E° =−0.35 V vs the Ag quasi- reference electrode. Accordingly, the MB+-(IV) conjugate was reduced to MBH-(IV), while applying, on a Hg-roughened Au electrode, a constant potential of−0.6 V vs the Ag wire reference electrode. The MBH-(IV) conjugate was oxidized to the original MB+-(IV) conjugate by applying a potential corresponding to−0.2 V vs the Ag wire.
I can understand that they did CV to find E’, but how the constant potential has been applied?
Dear Aisha Javed
I hope this message finds you well. I understand you are delving into electrochemical techniques, particularly cyclic voltammetry (CV) and constant potential electrolysis, and I wanted to share some insights that may help clarify their respective roles and applications.
In the context of your work with MB+-modified aptamers, CV is invaluable for mapping out the redox behavior of your system. By sweeping the potential, you can identify the specific voltages at which oxidation and reduction events occur, as evidenced by the quasi-reversible redox wave around -0.35 V. This foundational information is crucial for selecting the appropriate potentials for subsequent experiments.
Once these redox potentials are established, applying a constant potential—known as potentiostatic electrolysis—allows you to target specific redox transformations with precision. For example, holding the potential at -0.6 V vs Ag wire facilitates the reduction of MB+-(IV) to MBH-(IV), while -0.2 V enables its oxidation back. This approach is particularly useful when your goal is to drive a specific reaction to completion or to study the effects of sustained redox states, rather than observing dynamic changes as in CV.
If you have any further questions about when to use constant potential techniques versus cyclic voltammetry, or if you’d like to discuss your experimental design in more detail, please feel free to reach out. I’m happy to support your exploration of these methods.
Best regards,
Kaushik
Thankyou so much kaushik for reaching out, I am trying to reduce and oxidise MB, I am able to reduce the the MB by applying constant potential, but I am unable to oxidise it back. Can you guide me what wil be the case?
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