We identified 50 isolates from insect gut and found out through 16sr RNA sequencing. We have found seven groups such as Bacillus sps, Pseudomonas sps and remaining five groups along with other strains. We are interested to know the strain difference /polymorphism by softwares. We carried out DNAsp and pairwise distances. Moreover, I would like to know how rarefaction curve can work for this 50 isolates showing abundance and diversity. If so how to make this curve using applications or softwares. Can someone please guide me step by step /information’s where can I get. Thanks in advance
Thank you Dr.Jai Ghosh about curve. I am looking for any software gives strain difference and to know how to make rarefaction curve and related test details.
here is an example
http://www.mothur.org/wiki/Marine_community_analysis
and here is the command to get it done.
http://www.mothur.org/wiki/Rarefaction.single
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Biological Techniques