After culture the sample on PCA I got the colony like this. Can anyone identify upto species? the plate 4 is serial diluted.
I did PCR reaction without using any PCR kit . The result is not good enough. I got heavy low non specific band and probably primer dimer (picture attached). First, I did with annealing...
09 October 2019 1,944 4 View
I tried to isolate plasmid DNA from Agrobacterium tumefaciens using both manually and using kit and performed the electrophoresis on 0.5%, 0.8% and 1.0% agarose gel. The ladder showed the...
08 September 2019 2,256 2 View
For primer dimer there are many reasons. One is primer designing. Is there any way to solve the problem with the same primer that have been designed?
05 June 2018 3,800 2 View
After PCR followed by gel run every time I got this picture. Can anybody tell me why this happened and what will be the solution? In these two pictures, one gel had been run after putting 1kb...
04 May 2018 5,364 0 View
Any body can explain about the PCR and gradient PCR technology using an example?
11 December 2017 5,119 2 View
Like the title, anyone could suggest me some quality conferences in Biotechnology field hosted before Feb 2018? Thanks a lot!
09 October 2017 2,623 2 View
I designed some primer for my experiment and I do not know the actual length of the primers which I must need to know for getting the result. I got a manual with the primers supplied by the...
09 October 2017 2,070 4 View
While I am preparing WEB (Water extract molecule from Streptococcus pneumoniae) after 6 hours shaking it supposed to centrifuge in 10000rpm. But my centrifuge machine was shown error that is why I...
01 January 1970 2,957 2 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...
16 July 2024 5,047 0 View