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Questions related from Shahina Akter
I did PCR reaction without using any PCR kit . The result is not good enough. I got heavy low non specific band and probably primer dimer (picture attached). First, I did with annealing...
10 October 2019 1,900 4 View
I tried to isolate plasmid DNA from Agrobacterium tumefaciens using both manually and using kit and performed the electrophoresis on 0.5%, 0.8% and 1.0% agarose gel. The ladder showed the...
09 September 2019 2,186 2 View
After culture the sample on PCA I got the colony like this. Can anyone identify upto species? the plate 4 is serial diluted.
11 November 2018 4,651 0 View
For primer dimer there are many reasons. One is primer designing. Is there any way to solve the problem with the same primer that have been designed?
06 June 2018 3,735 2 View
After PCR followed by gel run every time I got this picture. Can anybody tell me why this happened and what will be the solution? In these two pictures, one gel had been run after putting 1kb...
05 May 2018 5,318 0 View
Any body can explain about the PCR and gradient PCR technology using an example?
12 December 2017 5,066 2 View
I designed some primer for my experiment and I do not know the actual length of the primers which I must need to know for getting the result. I got a manual with the primers supplied by the...
10 October 2017 2,013 4 View
Like the title, anyone could suggest me some quality conferences in Biotechnology field hosted before Feb 2018? Thanks a lot!
10 October 2017 2,558 2 View
While I am preparing WEB (Water extract molecule from Streptococcus pneumoniae) after 6 hours shaking it supposed to centrifuge in 10000rpm. But my centrifuge machine was shown error that is why I...
01 January 1970 2,897 2 View
