09 September 2014 3 9K Report

I am trying to design a primer pair for rbcL that could specifically amplify bryophytes using primer BLAST, but I'm confused. If I enter sequence/GenBank Accession of one template, for ex., Physcomitrella and choose "Specificity check enabled" and "Organism=Bryophyta", won't it get amplified for other mosses? 

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