I think it's better to add schematic of what I will do.
Red letters are what I want to ask. Thank you!
Stjepan Krešimir Kračun thank you!...
I made (cell-antibody) complex for target therapy purpose. and I have trouble identifying whether the antibody is properly conjugated on the cell surface. I used ac4mannaz for cell surface...
05 June 2019 5,392 4 View
As the title says, what makes the difference in antibody reactivity? I thought that if the antibody is same, they should all have same reactivity. But it isn't(according to the...
02 March 2019 5,108 9 View
I am working on treatment of gold nanoparticles (AuNPs) and gold nanocages on various cell lines (MSCs, Cancer cells, Cancer stem cells) I found out that the viability of the cells were decreased...
01 February 2019 7,465 3 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View