Can you help me in bioistatistic?

More Merve Gündoğdu's questions See All

Proliferation after fugene transfection?

Hi, I will do transfection with fugene but, I tried to enter three plasmids in cell. There is no selection part of plasmids, and I will try to plasmids enter to in cell one by one. How to do...

31 May 2023 1,940 3 View

Is it normal for the bands to disappear?

Hi, I did western and you can see my beta actin bands. I put 10 uL primary antibody for my interesting protein, but my professors said 25 uL should be. I donot understand. Wouldn't I have seen a...

11 April 2023 8,649 2 View

Why is there a band of my negative control?

Hello, I have band of my negative control. I am sure there is no contamination. I used new everything, sterile and change my gloves 5 times. I did negative control mix separately my samples. My...

02 January 2023 6,700 10 View

Do you have beta actin primer set seq?

Hello, Do you have beta actin primer sequences for qPCR? I will study with 3T3 (mouse fibroblast), Raji (Human B cell), U937 (Human monocyte), HEK293 (Human embryonic kidney). May you suggest...

30 November 2022 9,372 0 View

What should I do for cleaning my Sanger result?

Hello, Last week I asked my PCR results and I send Sanger seq. Well, there is one colony including mutation, I guess. How to cleaning my sample for Sanger? The Sanger result belongs to the second...

01 September 2022 7,808 4 View

Is there any problem my cDNA control results?

Hi everyone, First of all, thank you for attention and answers. I did reverse transcriptase PCR for obtain cDNA from RNA. I used 3 different cell line RNA and my results looks fine....

19 April 2022 5,610 0 View

Why is puc18 transformation not happening?

Hiii, I try to make mutations with QuikChange II XL Site-Directed Mutagenesis Kit. When I did first mutation, I got colonies and also mutation was succesfull. However, although I tried everything...

28 March 2022 1,919 3 View

Plasmid Selection with GFP?

Hi, I have a plasmid and designed site-directed mutagenesis primers, but my professor wants from me check plasmid with GFP after transformation? I donot know how to do that. Because our plasmid...

10 December 2021 6,245 4 View

In vitro mutagenesis primer design?

Hi, I have 2 specific variants and i want to create mutatation with mutagenesis primers. How can I design these primers? I found QuickChange Primer Design Tool but I couldnot use. Do you have any...

23 October 2021 5,620 3 View

In Silico Crispr?

Hi, Do you have any CRISPR/Cas9 system tool such as in silico PCR? I designed a crispr vector, before order it, I want to check. Thanks.

12 September 2021 3,069 0 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Fajar Awalia Yulianto

Fig 3 is a compilation of survival (and event, mortality in this case) cumulative curve. It derived from life table in a cohort (or trial) study.

Kannadasan Karuppaiah

In this graphs using only for SPSS software, if you have a raw data i can do it

Amr Adel Elnashar

We can recreate the data If you have the analysis output. All the data recorded in the survival table